ADVIA 2120 Red Blood Cell Analysis
A Standing Ovation
Clinicians and technologists alike will be standing and cheering for more.
The patented red cell technology utilized by the ADVIA 2120 provides detailed insight into erythropoietic disorders. This advanced cell separation provides a unique red blood cell differential.
Principles of Analysis
Iso-volumetric Sphering
The red cell reagent causes sphering and partial fixation of the cells. This sphering eliminates the shape of the cells as a variable, thereby reducing the potential for reporting erroneous results.
Flow Cytometry
Cells are analyzed using the principles of flow cytometry whereby the ADVIA 2120 sheath/rinse reagent encases the sample stream. This results in a single cell stream, which minimizes coincidence.
Monochromatic Light Scatter
Red cells are analyzed on the ADVIA 2120 using red laser light to measure both volume and hemoglobin concentrations on a cell by cell basis. The amount of light scattered at the low angle (2º-3º) is dependent on the volume of the cell. The amount of light scattered at the high angle (5º-15º) is related to the refractive index of the cell. This measurement provides the intracellular hemoglobin concentration of the red cell.
Red Cell Cytogram – MIE Map
The red cell cytogram is a graphical representation of these two measurements showing hemoglobin concentration on the x axis and volume on the y axis.
The non-linear relationship between these two measurements is described by the MIE Theory and can be replotted as a linear translation on the red cell erythrogram.
Red Cell Erythrogram
Using a pair of threshold gates on each axis, it is possible to define nine areas based on cell volume and hemoglobin content. This becomes the basis for the red cell morphology flagging on the ADVIA 2120.
The number of cells outside these thresholds gives an accurate percentage of microcytes, macrocytes, hypochromasia, and hyperchromasia.
This erythrogram provides sensitive and specific detection of minority red cell populations, which assists in differentiating between such disease states as iron deficiency anemia, thalassemias, and other RBC abnormalities.
Red Cell Morphology Flags:
- MICRO • HYPER • RBCF – RBC Fragments
- MACRO • HYPO • RBCG – RBC Ghosts
- ANISO • HCVAR
Summary of ADVIA 2120 Red Blood Cell Analysis:
- Direct measurements of intracellular hemoglobin provide sensitive and specific red cell morphology for the accurate and early detection of minority cell populations.
- Unique % Hypo* parameter for differentiating between iron deficiency, thalassemia, and hemoglobinopathies leads to appropriate and early treatment.
- % Hypo parameter assists clinicians in the monitoring and optimizing of EPO and iron therapies.
- % Hyper* parameter assists with the detection of abnormalities such as spherocytosis and sickle cell anemia.
- Dual hemoglobin measurements, spectrophotometric and cellular, ensure accurate HGB results.
No Doubts. Higher Confidence. Better Efficiency!
* % Hypo and % Hyper assays are for research use only, not for use in diagnostic procedures in the U.S.
