ADVIA 2120 Reticulocytes
Simply Spellbinding
You won’t be able to tear yourself away from the ADVIA 2120 system's reticulocyte capabilities.
By measuring both size and hemoglobin content, the ADVIA 2120 not only determines the rate of erythropoiesis, but also the effectiveness of reticulocytes in terms of their heme synthesis capabilities. In other words, reticulocytes provide a "real time" assessment of erythropoietic activity.
Principles of Analysis
Sphering and Staining
The reticulocyte technology utilized by the ADVIA 2120 is based on that of the red cell method, again utilizing the MIE Theory of light scatter, with the addition of a nucleic acid fluorescent dye.
The cells are isovolumetrically sphered, stained, and subsequently analyzed by the laser optical assembly.
Cellular Analysis by Light Scatter
The cell volume is plotted on the vertical axis, with increasing absorption plotted on the horizontal axis. The more staining in the cell, the further it appears along the axis from left to right. The threshold between mature red blood cells and reticulocytes is obtained through statistical analysis. The reticulocyte population is then subdivided into three populations of low, medium, and high absorption. This analysis is performed on approximately 50,000 red blood cells, providing both an absolute reticulocyte count and a percent reticulocyte.
Scatter Cytograms
Retic Scatter Cytogram
This scatter cytogram is the graphical representation of two light-scatter measurements. The high-angle, low-gain light scatter is plotted on the x axis, while the low-angle, low-gain light scatter is plotted along the y axis. The MIE Map is superimposed on the cytogram, indicating volume and hemoglobin concentration information.Cells identified as mature red blood cells are shown in red, while cells identified as reticulocytes are colored blue. This map shows reticulocytes as cells that are larger than mature red cells with lower hemoglobin concentration.
Retic Scatter ABS Cytogram
The Retic scatter ABS cytogram is the graphical representation of absorption and light-scatter measurements. Absorption (cell maturation) is plotted along the x axis, while volume (cell size) is plotted along the y axis.
Summary of ADVIA 2120 Reticulocyte Analysis:
- Fully automated reticulocyte count and percent.
- Unique, clinically relevant, and widely accepted parameter, CHr™, is an effective tool in managing the anemias associated with chronic renal failure.
- CHr is effective in monitoring and determining the effectiveness of costly EPO therapy, widely used in the treatment of anemia, and now with indications in pediatrics and oncology.
- Reduces cost of EPO therapy.
No Doubts. Higher Confidence. Better Efficiency!
