A Stellar Performance

Even your toughest critics will be awed by this new classic.

The ADVIA 2120 Hematology System uses a combination of light scatter, cytochemical staining, and nuclear density on two independent channels to measure the total and differential white cell counts. Benefit from technology which utilizes peroxidase staining – the gold standard for differential testing.

Principles of Analysis

Flow Cytometry
Cells are analyzed using the principles of flow cytometry whereby the ADVIA 2120 sheath/rinse reagent encases the sample stream. This results in a single cell stream, which minimizes coincidence. Measurement of total white cell count and differential cluster analysis identify each cell according to its size and light absorption properties. The exact number of cells within each cluster is counted. Moving threshold cluster analysis is performed in order to adapt to changes in cell characteristics.

The Peroxidase Method

This is the primary differential method on the ADVIA 2120, and additionally provides a secondary total white cell count. This back-up white cell count acts as an internal QC check to monitor sample integrity.
Cytochemical reaction is a two-stage chemistry method utilizing the intracellular myeloperoxidase enzyme to differentiate cells using stain and size characteristics. The cells are analyzed by the addition of the peroxidase enzyme substrate. Absorbance of the white light from the tungsten light source is a measure of the intensity of the peroxidase reaction: neutrophils, monocytes, and eosinophils are peroxidase positive, whereas lymphocytes and basophils are peroxidase negative.
In addition to the standard five-population differential, the ADVIA 2120 reports an additional population called large unstained cells (LUCs). These cells are usually virally activated lymphocytes, plasma cells, hairy cells, pediatric lymphocytes, or peroxidase negative blasts.

1 Noise
2 Nucleated Red Blood Cells
3 Platelet Clumps
4 Lymphocytes and Basophils
5 Large Unstained Cells
6 Monocytes
7 Neutrophils
8 Eosinophils

Cells identified as mature red blood cells are shown in red, while cells identified as reticulocytes are colored blue. This map shows reticulocytes as cells that are larger than mature red cells with lower hemoglobin concentration.

The Basophil/Lobularity Method

The basophil method provides the primary total white cell count on the ADVIA 2120. The BASO reagent lyses the red cells, platelets, and the cytoplasm of all white cell types except basophils. The BASO cytogram uses cluster analysis to identify and count the cells and the nuclei in each population based on their position, area, and density.

1 Noise
2 Blast cell nuclei
3 Mononuclear WBCs (Monocyte and Lymphocyte nuclei)
4 Basophils
5 Baso Suspect
6 Saturation
7 Polymorphonuclear WBCs (Neutrophil and Eosinophil nuclei)

High-angle light scatter (nuclear configuration) is plotted on the x axis. Low-angle light scatter (cell size) is plotted on the y axis.

White Cell Morphology Flags:

ATYPS – Atypical Lymphocytes
NRBC – Nucleated Red Blood Cells
Blasts – Suspected Blasts
LS – Left Shift
IG – Immature Granulocytes

Summary of ADVIA 2120 White Blood Cell Technology

• Dual technology provides accurate, specific flagging that lowers manual review rates.
• Reduced differential reject rates.
• Diagnostic cytograms assist with rapid diagnosis and result validation.
• Specific cytochemical differentiation of myeloid and lymphoid cells.
• Unique six-parameter differential with LUC population provides additional clinical information.
• Accurate differentials on leucopenic and aged samples.
• Extended linearity of 400 x 1000/µL minimizes the need for dilutions.

No Doubts. Higher Confidence. Better Efficiency!