VERSANT® HCV RNA 3.0 Assay (bDNA)
Consistent Genotype Results
VERSANT HCV RNA 3.0 Assay (bDNA) accurately quantitates all HCV RNA genotypes.
- Equivalent detection of genotypes 1-6
Detection of Genotypes 1-6
Fig 1. Relative recovery of non-1a HCV genotype transcripts compared to that of the genotype 1a transcript. Transcripts were tested at two concentrations.1
Accurate Quantitation
VERSANT HCV RNA 3.0 Assay (bDNA) accurately quantitates throughout its broad dynamic range.
- When compared to the VERSANT assay, the COBAS Amplicor™ Monitor 2.0 assay under-quantitates a substantial number of results.2, 3, 4 This means that viral load baseline values for some patients may be significantly higher than reported by Amplicor
- Even after dilution, some PCR specimens are under-quantitated as much as 1-log when compared to bDNA
Quantitation Comparisons: bDNA vs. PCR
Fig 2. Comparative evaluations based on three studies comparing Siemens Healthcare Diagnostics and Roche's™ HCV viral load assays demonstrated that they were quite dissimilar within higher-end range as determined by the bDNA assay.2, 3, 4 PCR specimens within the lower range exhibited discrepancies of as much as 78,576 IU/mL.2
References
1. Elbeik, T et al. 2004. Multicenter Evaluation of the Performance Characteristics of the Siemens VERSANT HCV RNA 3.0 Assay (bDNA). JCM, 42(2); 563-569.
2. Germer et al. 2002. Comparative Evaluation of the VERSANT HCV RNA 3.0, QUANTIPLEX HCV RNA 2.0, and COBAS AMPLICOR HCV MONITOR Version 2.0 Assays for Quantification of Hepatitis C Virus RNA in Serum. JCM 40(2); 495-200.
3. Konnick, E et al. 2002. Performance Characteristics of the COBAS Amplicor Hepatitis C Virus (HCV) Monitor, Version 2.0, International Unit Assay and the National Genetics Institute HCV Superquant Assay. JCM, 40(3); 768-773.
4. Beld, M et al. 2002. Performance of the New Siemens VERSANT HCV RNA 3.0 Assay for Quantitation of Hepatitis C Virus RNA in Plasma and Serum: Conversion to International Units and Comparison with the Roche COBAS Amplicor HCV Monitor, Version 2.0, Assay. JCM, 40(3); 788-793.
